Fundamentals of Light Microscopy and Electronic Imaging

This practical book presents the fundamentals of light microscopy, including the basics of microscope design, image formation, and camera function. Moreover, researchers will learn how to acquire electronic images and perform image processing. The author provides instructions on the latest techniques involving confocal laser scanning microscopy, digital CCD microscopy, as well as a chapter covering issues related to image processing for scientific publication. Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as: Illuminators, filters, and isolation of specific wavelengths Phase contrast and differential interference contrast Properties of polarized light and polarization microscopy Fluorescence and confocal laser scanning microscopy Digital CCD microscopy and image processing Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions. Both experienced and novice microscopists will find Fundamentals of Light Microscopy and Electronic Imaging an essential resource.